Effluent Fluid Culture & Gram Stain
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- One set of Bactec Culture Bottles (1 aerobic and 1 anaerobic) and 1 Tall Yellow Top Tube are used for each Fluid Culture ordered.
and
- Disinfect the Bactec Culture Bottles by wiping the tops of the bottles with a 70% Alcohol Prep Pad and leaving the pad on top of the bottle until ready to inoculate.
- DO NOT USE ANY FORM OF IODINE TO DISINFECT THE TOPS OF THE BOTTLES. It may affect the integrity of the rubber top of the culture bottle, causing false positive results
- Attach Safety Needle to Holder.
- Remove green needle cover and insert needle into medication port.
- Insert the top of the aerobic Bactec Culture Bottle into the Holder. This will pierce the stopper and allow the bottle to automatically fill.
- Allow fluid to fill until 8-10 mL collects.
- Remove the aerobic Bactec Culture Bottle from the Holder.
- Insert the top of the anaerobic Bactec Culture Bottle into the Holder (you may use the same Holder).
- Allow fluid to fill until 8-10 mL collects.
- Insert the stopper of the Tall Yellow Top Tube into the Holder. This will pierce the stopper and allow the tube to automatically fill (one tube per test ordered).
- Allow fluid to fill until flow ceases.
- Use the bar code labels with patient’s name and ID to identify each Bactec Culture Bottle and the Tall Yellow Top Tube.
Note: Do not place the patient’s bar code label over the bottle’s bar code label. - Hold at room temperature.