HIV 1,2 Ab/Ag w/Reflex Antibody Diff. & RNA
| Alternate Names | HIV Screen, Fourth Generation HIV, 4th Generation HIV |
| Ordering Recommendation | May be used to help diagnose HIV-1 and HIV-2 infection, including acute infection, and to differentiate HIV-1 from HIV-2.
This test allows detection of acute HIV-1 infection based on the presence of p24 antigen, before seroconversion occurs, allowing for earlier diagnosis than with previous tests. It can also detect HIV-1 and HIV-2 post-seroconversion, based on antibodies. If the HIV-1/HIV-2 antigen/antibody test is positive, an antibody-based HIV-1/HIV-2 test is performed to confirm infection and identify whether it is caused by HIV-1 or HIV-2. However, the HIV-1/HIV-2 differentiation test will be negative during acute infection (prior to development of specific antibodies). If the antibody-based HIV-1/HIV-2 test is negative, the blood specimen will be reflex-tested for the presence of HIV-1 and HIV-2 RNA to identify patients with acute HIV infection |
| Specimen | Lithium Heparin Plasma (HIV 1,2 Ab/Ag Screen) EDTA Plasma (Reflex Antibody Differentiation & RNA) |
| Container Type | Green Top Tube
If only HIV is ordered, collect an additional Lavender Top Tube
|
| Tests Included | HIV 1,2 Ag/Ab Screen
If HIV 1,2 Ag/Ab Screen is preliminarily Reactive, then HIV-1/HIV-2 Antibody Differentiation will be reflexed and performed at an additional charge (CPT codes: 86701, 86702). If HIV-1/HIV-2 Antibody Differentiation is Negative or Indeterminate, then HIV-1/HIV-2 RNA, Qualitative PCR will be performed at an additional charge (CPT codes: 87535, 87538) |
| Methodology | Chemiluminescent Sandwich Immunoassay
Test performed using Atellica IM HIV Ab/Ag Combo (CHIV) chemiluminescent assay. Values obtained with different assay methods cannot be used interchangeably. |
| Reference Range | HIV 1,2 Ag/Ab Screen: Non-Reactive
Reflex Antibody Differentiation & RNA: See patient report |
| Stability | HIV 1,2 Ag/Ab Screen: 14 days
Reflex Antibody Differentiation & RNA: 6 days |
| Transport Temp | Refrigerate 2°-8°C |
| Instructions | If only HIV is ordered, collect an additional lavender top for possible reflex testing.
All preliminary Reactive HIV Screen results will be submitted for additional confirmatory testing, which must be considered in making a diagnosis related to HIV infection in conjunction with clinical evaluation. |
| Performing Lab | HIV 1,2 Ag/Ab Screen: Ascend Clinical
Reflex Antibody Differentiation & RNA: |
| Days Performed | HIV 1,2 Ag/Ab Screen: Tues, Fri
Reflex Antibody Differentiation & RNA: Varies (test based) |
| Report Available | HIV 1,2 Ag/Ab Screen: 1-5 days
Reflex Antibody Differentiation & RNA: 2-5 days (beyond HIV 1,2 Ag/Ab Screen) |
| NY State Available | Yes |
| LOINC Codes | 56888-1, 68961-2, 81641-3, 25835-0, 69353-1 |
| CPT Codes | 86703, 86701, 86702, 87535, 87538 |
